Medium M71

[所属分类:培养基配方] [发布时间:2021-5-20] [发布人:网站管理员2] [阅读次数:] [返回]

Medium M71

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................20.0g
Peptone........................................................................................10.0g
Glucose .........................................................................................5.0g
H 3 BO 3 ...........................................................................................1.0g
Pancreatic digest of casein............................................................1.0g
Cycloheximide............................................................................0.05g
2,3,5-Triphenyltetrazolium·HCl solution.................................10.0mL
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
2,3,5-Triphenyltetrazolium·HCl Solution:
Composition per 10.0mL:
2,3,5-Triphenyltetrazolium·HCl..................................................0.05g
Preparation of 2,3,5-Triphenyltetrazolium·HCl Solution:
Add 2,3,5-triphenyltetrazolium·HCl to distilled/deionized water and
bring volume to 10.0mL. Mix thoroughly. Autoclave for 15 min at 15
psi pressure–121°C.
Preparation of Medium: Add components, except 2,3,5-triphenyl-
tetrazolium·HCl solution, to distilled/deionized water and bring vol-
ume to 990.0mL. Mix thoroughly. Gently heat and bring to boiling.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Aseptically add 10.0mL of sterile 2,3,5-triphenyltetrazolium·HCl solu-
tion. Mix thoroughly. Pour into sterile Petri dishes.
Use: For the selective isolation and cultivation of Pseudomonas syrin-
gae.