Medium for Nitrite Oxidizers, Marine

[所属分类:培养基配方] [发布时间:2021-5-20] [发布人:网站管理员2] [阅读次数:] [返回]

Medium for Nitrite Oxidizers, Marine

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

MgSO 4 ·7H 2 O................................................................................0.1g
NaNO 2 .........................................................................................0.07g
CaCl 2 ·2H 2 O................................................................................6.0mg
K 2 HPO 4 ....................................................................................1.74mg
Chelated iron..............................................................................1.0mg
MnCl 2 ·4H 2 O..............................................................................66.0μg
Na 2 MoO 4 ·2H 2 O ........................................................................30.0μg
ZnSO 4 ·7H 2 O.............................................................................30.0μg
CuSO 4 ·5H 2 O...............................................................................6.0μg
CoCl 2 ·6H 2 O ................................................................................0.6μg
Seawater.................................................................................700.0mL
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the isolation, cultivation, and enrichment of marine nitrate-
oxidizing bacteria.