Medium with Phenanthrene (DSMZ Medium 457b)

[所属分类:培养基配方] [发布时间:2021-5-20] [发布人:网站管理员2] [阅读次数:] [返回]

Medium with Phenanthrene
(DSMZ Medium 457b)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Na 2 HPO 4 .....................................................................................2.44g
KH 2 PO 4 .......................................................................................1.52g
(NH 4 ) 2 SO 4 .....................................................................................0.5g
MgSO 4 ·7H 2 O................................................................................0.2g
Tween 80.......................................................................................0.2g
CaCl 2 ·2H 2 O.................................................................................0.05g
Phenanthrene solution..............................................................50.0mL
Trace elements solution SL-4 ..................................................10.0mL
pH 6.9 ± 0.2 at 25°C
Trace Elements Solution SL-4:
Composition per liter:
EDTA............................................................................................0.5g
FeSO 4 ·7H 2 O..................................................................................0.2g
Trace elements solution SL-6 ................................................100.0mL
Preparation of Trace Elements Solution SL-4: Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Trace Elements Solution SL-6:
Composition per liter:
H 3 BO 3 ...........................................................................................0.3g
CoCl 2 ·6H 2 O ..................................................................................0.2g
ZnSO 4 ·7H 2 O.................................................................................0.1g
MnCl 2 ·4H 2 O ...............................................................................0.03g
Na 2 MoO 4 ·H 2 O............................................................................0.03g
NiCl 2 ·6H 2 O.................................................................................0.02g
CuCl 2· ·2H 2 O................................................................................0.01g
Preparation of Trace Elements Solution SL-6: Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Adjust pH to 3.4.
Phenanthrene Solution:
Composition per liter:
Phenanthrene.................................................................................2.0g
Preparation of Phenanthrene Solution: Add phenanthrene to
1.0L acetone. Mix thoroughly. Filter sterilize using a cellulose filter
membrane.
Preparation of Medium: Add components, except phenanthrene so-
lution, to 1.0L distilled/deionized water. Adjust pH to 6.9. Autoclave for
15 min at 15 psi pressure–121°C. Cool to room temperature. Add an al-
iquot of the phenanthrene solution to a sterile flask so that the final con-
centration will be 0.1g/L phenanthrene, and let the acetone evaporate.
Aseptically add sterile medium to the crystal-layered flask.
Use: For the cultivation of phenanthrene-utilizing Sphingomonas sp.
(Pseudomonas paucimobilis), Pseudomonas frederiksbergensis, and other
bacteria.