Medium R

[所属分类:培养基配方] [发布时间:2021-5-20] [发布人:网站管理员2] [阅读次数:] [返回]

Medium R

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Na 2 S 2 O 3 ·5H 2 O..............................................................................5.0g
KNO 3 ............................................................................................2.0g
MgCl 2 ·6H 2 O..................................................................................0.5g
NH 4 Cl ...........................................................................................0.5g
KH 2 PO 4 solution......................................................................10.0mL
NaHCO 3 solution.....................................................................10.0mL
FeSO 4 ·7H 2 O solution...............................................................10.0mL
pH 7.0 ± 0.2 at 25°C
KH 2 PO 4 Solution:
Composition per 10.0mL:
KH 2 PO 4 .........................................................................................2.0g
Preparation of KH 2 PO 4 Solution: Add KH 2 PO 4 to distilled/de-
ionized water and bring volume to 10.0mL. Mix thoroughly. Filter ster-
ilize.
NaHCO 3 Solution:
Composition per 10.0mL:
NaHCO 3 ........................................................................................1.0g
Preparation of NaHCO 3 Solution: Add the NaHCO 3 to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
FeSO 4 ·7H 2 O Solution:
Composition per 10.0mL:
FeSO 4 ·7H 2 O.............................................................................10.0mg
Preparation of FeSO 4 ·7H 2 O Solution: Add the FeSO 4 ·7H 2 O to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Filter sterilize.
Preparation of Medium: Add components—except KH 2 PO 4 solu-
tion, NaHCO 3 solution, and FeSO 4 ·7H 2 O solution—to tap water and
bring volume to 970.0mL. Mix thoroughly. Gently heat until dissolved.
Adjust pH to 7.0. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 45°–50°C. Aseptically add 10.0mL of sterile KH 2 PO 4 solution,
10.0mL of NaHCO 3 solution, and 10.0mL of FeSO 4 ·7H 2 O solution.
Mix thoroughly. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation of Thiobacillus denitrificans.