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培养基配方
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Medium for Sulfate Reducers (Postgate’s Medium for Sulfate Reducers) (ATCC Medium 1283)
[所属分类:培养基配方] [发布时间:2021-5-20] [发布人:网站管理员2] [阅读次数:] [返回]
Medium for Sulfate Reducers
(Postgate’s Medium for Sulfate Reducers)
(ATCC Medium 1283)
Part C.....................................................................................100.0mL
Part D.......................................................................................10.0mL
Part E .......................................................................................10.0mL
Part F........................................................................................10.0mL
Part B.........................................................................................1.0mL
pH 7.7 ± 0.2 at 25°C
Part A:
Composition per 869.0mL:
Na 2 SO 4 ..........................................................................................3.0g
NaCl..............................................................................................1.0g
KCl................................................................................................0.5g
MgCl 2 ·6H 2 O .................................................................................0.4g
NH 4 Cl ...........................................................................................0.3g
KH 2 PO 4 .........................................................................................0.2g
CaCl 2 ·2H 2 O ................................................................................0.15g
Preparation of Part A: Add components to distilled/deionized wa-
ter and bring volume to 869.0mL. Mix thoroughly. Prepare and auto-
clave part A under 90% N 2 + 10% CO 2 . Autoclave for 15 min at 15 psi
pressure–121°C. Cool to room temperature.
Part B:
Composition per liter:
FeCl 2 ·4H 2 O...................................................................................1.5g
CoCl 2 ·6H 2 O................................................................................0.19g
MnCl 2 ·4H 2 O .................................................................................0.1g
ZnCl 2 ...........................................................................................0.07g
H 3 BO 3 .........................................................................................0.06g
Na 2 MoO 4 ·2H 2 O ..........................................................................0.04g
NiCl 2 ·6H 2 O.................................................................................0.02g
CuCl 2 ·2H 2 O ................................................................................0.02g
HCl, 25% .................................................................................10.0mL
Preparation of Part B: Add the FeCl 2 ·4H 2 O to the HCl. Add dis-
tilled/deionized water and bring volume to 1.0L. Add remaining com-
ponents. Mix thoroughly. Autoclave under 100% N 2 for 15 min at 15
psi pressure–121°C. Cool to room temperature.
Part C:
Composition per 100.0mL:
NaHCO 3 ........................................................................................5.0g
Preparation of Part C: Add the NaHCO 3 to distilled/deionized wa-
ter and bring volume to 100.0mL. Mix thoroughly. Filter sterilize. Gas
with 90% N 2 + 10% CO 2 to remove residual O 2 .
Part D:
Composition per 10.0mL:
Sodium butyrate............................................................................0.7g
Sodium caproate ...........................................................................0.3g
Sodium octanoate........................................................................0.15g
Preparation of Part D: Add components to distilled/deionized wa-
ter and bring volume to 10.0mL. Mix thoroughly. Autoclave under
100% N 2 for 15 min at 15 psi pressure–121°C. Cool to room tempera-
ture.
Part E:
Composition per 10.0mL:
Yeast extract..................................................................................1.0g
Thiamine·HCl .........................................................................100.0μg
p-Aminobenzoic acid................................................................40.0μg
D(+)-Biotin ...............................................................................10.0μg
Preparation of Part E: Add components to distilled/deionized water
and bring volume to 10.0mL. Mix thoroughly. Autoclave under 100% N 2
for 15 min at 15 psi pressure–121°C. Cool to room temperature.
Part F:
Composition per 10.0mL:
Na 2 S·9H 2 O....................................................................................0.4g
Preparation of Part F: Add Na 2 S·9H 2 O to distilled/deionized wa-
ter and bring volume to 10.0mL. Mix thoroughly. Autoclave under
100% N 2 for 15 min at 15 psi pressure–121°C. Cool to room tempera-
ture.
Preparation of Medium: To 869.0mL of sterile cooled part A,
aseptically add the remaining sterile solutions in the following order:
part B, part C, part D, part E, and part F. Mix thoroughly. Adjust pH to
7.7. Anaerobically distribute under 80% N 2 + 20% CO 2 into sterile
tubes or flasks.
Use: For the cultivation and maintenance of Desulfovibrio baarsii and
Desulfovibrio sapovorans.
(Postgate’s Medium for Sulfate Reducers)
(ATCC Medium 1283)
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Part A.....................................................................................869.0mLPart C.....................................................................................100.0mL
Part D.......................................................................................10.0mL
Part E .......................................................................................10.0mL
Part F........................................................................................10.0mL
Part B.........................................................................................1.0mL
pH 7.7 ± 0.2 at 25°C
Part A:
Composition per 869.0mL:
Na 2 SO 4 ..........................................................................................3.0g
NaCl..............................................................................................1.0g
KCl................................................................................................0.5g
MgCl 2 ·6H 2 O .................................................................................0.4g
NH 4 Cl ...........................................................................................0.3g
KH 2 PO 4 .........................................................................................0.2g
CaCl 2 ·2H 2 O ................................................................................0.15g
Preparation of Part A: Add components to distilled/deionized wa-
ter and bring volume to 869.0mL. Mix thoroughly. Prepare and auto-
clave part A under 90% N 2 + 10% CO 2 . Autoclave for 15 min at 15 psi
pressure–121°C. Cool to room temperature.
Part B:
Composition per liter:
FeCl 2 ·4H 2 O...................................................................................1.5g
CoCl 2 ·6H 2 O................................................................................0.19g
MnCl 2 ·4H 2 O .................................................................................0.1g
ZnCl 2 ...........................................................................................0.07g
H 3 BO 3 .........................................................................................0.06g
Na 2 MoO 4 ·2H 2 O ..........................................................................0.04g
NiCl 2 ·6H 2 O.................................................................................0.02g
CuCl 2 ·2H 2 O ................................................................................0.02g
HCl, 25% .................................................................................10.0mL
Preparation of Part B: Add the FeCl 2 ·4H 2 O to the HCl. Add dis-
tilled/deionized water and bring volume to 1.0L. Add remaining com-
ponents. Mix thoroughly. Autoclave under 100% N 2 for 15 min at 15
psi pressure–121°C. Cool to room temperature.
Part C:
Composition per 100.0mL:
NaHCO 3 ........................................................................................5.0g
Preparation of Part C: Add the NaHCO 3 to distilled/deionized wa-
ter and bring volume to 100.0mL. Mix thoroughly. Filter sterilize. Gas
with 90% N 2 + 10% CO 2 to remove residual O 2 .
Part D:
Composition per 10.0mL:
Sodium butyrate............................................................................0.7g
Sodium caproate ...........................................................................0.3g
Sodium octanoate........................................................................0.15g
Preparation of Part D: Add components to distilled/deionized wa-
ter and bring volume to 10.0mL. Mix thoroughly. Autoclave under
100% N 2 for 15 min at 15 psi pressure–121°C. Cool to room tempera-
ture.
Part E:
Composition per 10.0mL:
Yeast extract..................................................................................1.0g
Thiamine·HCl .........................................................................100.0μg
p-Aminobenzoic acid................................................................40.0μg
D(+)-Biotin ...............................................................................10.0μg
Preparation of Part E: Add components to distilled/deionized water
and bring volume to 10.0mL. Mix thoroughly. Autoclave under 100% N 2
for 15 min at 15 psi pressure–121°C. Cool to room temperature.
Part F:
Composition per 10.0mL:
Na 2 S·9H 2 O....................................................................................0.4g
Preparation of Part F: Add Na 2 S·9H 2 O to distilled/deionized wa-
ter and bring volume to 10.0mL. Mix thoroughly. Autoclave under
100% N 2 for 15 min at 15 psi pressure–121°C. Cool to room tempera-
ture.
Preparation of Medium: To 869.0mL of sterile cooled part A,
aseptically add the remaining sterile solutions in the following order:
part B, part C, part D, part E, and part F. Mix thoroughly. Adjust pH to
7.7. Anaerobically distribute under 80% N 2 + 20% CO 2 into sterile
tubes or flasks.
Use: For the cultivation and maintenance of Desulfovibrio baarsii and
Desulfovibrio sapovorans.
