Medium for Treponema pectinovorum

[所属分类:培养基配方] [发布时间:2021-5-20] [发布人:网站管理员2] [阅读次数:] [返回]

Medium for Treponema pectinovorum

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Polypeptone™ ..............................................................................5.0g
Heart infusion broth......................................................................5.0g
Yeast extract..................................................................................5.0g
NaCl..............................................................................................5.0g
K 2 HPO 4 .........................................................................................2.0g
(NH 4 ) 2 SO 4 ....................................................................................2.0g
Agar..............................................................................................1.0g
Pectin............................................................................................0.8g
L- Cysteine·HCl·H 2 O ...................................................................0.68g
Rumen fluid...........................................................................500.0mL
Resazurin (25.0 mg/100.0mL water).........................................4.0mL
pH 7.0–7.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Prepare and distribute anaerobically
under 90% N 2 + 10% CO 2 . Mix thoroughly. Adjust pH to 7.0–7.2. Dis-
tribute into screw-capped tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C.
Use:  For the cultivation and maintenance of Treponema pectinovo-
rum.