Melin–Norkrans Medium (MN)

[所属分类:培养基配方] [发布时间:2021-5-20] [发布人:网站管理员2] [阅读次数:] [返回]

Melin–Norkrans Medium
(MN)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1001.2mL:

Agar............................................................................................15.0g
Glucose.......................................................................................10.0g
Malt extract...................................................................................2.8g
KH 2 PO 4 .........................................................................................0.5g
(NH 4 ) 2 HPO 4 ................................................................................0.25g
MgSO 4 ·7H 2 O..............................................................................0.15g
CaCl 2 ...........................................................................................0.05g
NaCl..........................................................................................0.025g
Thiamine....................................................................................0.1mg
Biotine....................................................................................0.005mg
Oligo solution..........................................................................1.66mL
FeCl 3  solution.............................................................................1.2mL
FeCl 3 Solution:
Composition per 10.0mL:
FeCl 3 .............................................................................................1.0g
Preparation of FeCl 3 Solution: Add FeCl 3  to distilled/deionized
water and bring volume to 10.0mL. Mix thoroughly.
Oligo Solution:
Composition per 1.66mL:
Lilly and Barnett solution..........................................................1.0mL
Hoagland 1% solution..............................................................0.66mL
Hoagland Solution:
Composition per 100.0mL:
Fe(NO 3 ) 3 ·9H 2 OH 3 BO 3 ...............................................................2.86g
MnCl 2 ..........................................................................................1.81g
ZnSO4·7H 2 O.............................................................................. 0.22g
CuSO 4 ·5H 2 O.............................................................................. 0.08g
H 2 MoO 4 ·H 2 0.............................................................................. 0.01g
Preparation of Hoagland Solution: Add components to distilled/
deionized water and bring volume to 100.0mL. Mix thoroughly.
Lilly and Barnett Solution:
Composition per 100.0mL:
Fe(NO 3 ) 3 ·9H 2 O......................................................................723.5mg
ZnSO 4 ·7H 2 O..........................................................................439.8mg
Preparation of Lilly and Barnett Solution: Add components to
distilled/deionized water and bring volume to 100.0mL. Mix thorough-
ly.
Preparation of Oligo Solution: Combine 1.0mL of Lilly and Bar-
nett solution and 0.66mL of 1% Hoagland solution.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use:  For the cultivation of Agaricus xanthoderma, Agaricus mac-
rosporus, Antrodia serialis, Armillaria mellea, Auricularia fuscosuc-
cinea, Boletinellus merulioides, Boletus leucophaeus, Cephaliophora
irregularis, Circinella umbellata, Kuehneromyces mutabilis, Laccaria
laccata, Lentinus tigrinus, Lenzites betulina, Leucogyrophana mol-
lusca, Lycoperdon foetidum, Macrolepiota rhacodes, Macrolepiota
procera, Pholiota lenta, Phoma exigua, and many other fungi.