Methanobacterium formicicum Medium

[所属分类:培养基配方] [发布时间:2021-5-21] [发布人:网站管理员2] [阅读次数:] [返回]

Methanobacterium formicicum Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCH 3 CO 2 ....................................................................................2.0g
(NH 4 ) 2 SO 4 ..................................................................................1.48g
NaCl............................................................................................0.45g
L -Cysteine·HCl·H 2 O...................................................................0.27g
CaCl 2 ·2H 2 O ................................................................................0.06g
Fe(NH 4 )(SO 4 ) 2 ............................................................................0.06g
MgSO 4 .....................................................................................45.0mg
Na 2 MoO 4 .................................................................................24.0mg
K 2 HPO 4 ....................................................................................21.0mg
KH 2 PO 4 ....................................................................................21.0mg
Resazurin...................................................................................1.0mg
Na 2 SeO 3 .....................................................................................0.2mg
Na 2 S·9H 2 O solution...................................................................1.0mL
pH 6.8 ± 0.2 at 25°C
Na 2 S·9H 2 O Solution:
Composition per 100.0mL:
Na 2 S·9H 2 O..................................................................................25.0g
Preparation of Na 2 S·9H 2 O Solution:  Bring 100.0mL of dis-
tilled/deionized water to boiling. Cool to room temperature while
sparging with 100%N 2 . Add Na 2 S·9H 2 O to the 100.0mL of anaerobic
water. Mix thoroughly. Distribute into serum bottles fitted with butyl
rubber stoppers and aluminum seals. Do not grease stoppers. Auto-
clave for 20 min at 15 psi pressure–121°C.
Preparation of Medium: Add components, except Na 2 S·9H 2 O so-
lution, to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Adjust pH to 6.8 with concentrated HCl. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Cool medi-
um while sparging with 100% N 2 . Prior to inoculation, aseptically and
anaerobically add 1.0mL of sterile Na 2 S·9H 2 O solution per liter of me-
dium. Repeat the addition of 1.0mL of sterile Na 2 S·9H 2 O solution per
liter of medium every 48 hr during growth.
Use:  For the cultivation of Methanobacterium formicicum.