Methanobacterium wolfei Medium

[所属分类:培养基配方] [发布时间:2021-5-24] [发布人:网站管理员2] [阅读次数:] [返回]

Methanobacterium wolfei Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Na 2 CO 3 .........................................................................................4.0g
Sodium acetate·3H 2 O....................................................................1.0g
L -Cysteine·HCl..............................................................................0.5g
NaCl..............................................................................................0.5g
Na 2 S·9H 2 O....................................................................................0.5g
K 2 HPO 4 .......................................................................................0.26g
KH 2 PO 4 .......................................................................................0.26g
(NH 4 ) 2 SO 4 ...................................................................................0.26g
MgSO 4 ·7H 2 O................................................................................0.1g
CaCl 2 ·2H 2 O.................................................................................0.07g
NaWO 4 .......................................................................................2.6mg
FeSO 4 .........................................................................................2.0mg
Resazurin ...................................................................................1.0mg
Vitamin solution.......................................................................10.0mL
Trace elements solution ...........................................................10.0mL
pH 7.2 ± 0.2 at 25°C
Trace Elements Solution:
Composition per liter:
Nitrilotriacetic acid.......................................................................9.5g
MgCl 2 ..........................................................................................4.06g
FeCl 2 ...........................................................................................0.99g
NiCl 2 ·6H 2 O.................................................................................0.12g
Na 2 MoO 4 ...................................................................................0.024g
CoCl 2 ·6H 2 O ..............................................................................0.023g
Preparation of Trace Elements Solution:  Add nitrilotriacetic
acid to 500.0mL of distilled/deionized water. Adjust pH to 6.5 with
KOH. Add remaining components one at a time. Add distilled/deion-
ized water to 1.0L. Mix thoroughly. Sparge with 100% N 2 . Adjust pH
to 7.0 with 10N NaOH.
Vitamin Solution:
Composition per liter:
Calcium  DL -pantothenate..............................................................5.0g
Vitamin B 12 ...................................................................................0.1g
Pyridoxine·HCl........................................................................10.0mg
p-Aminobenzoic acid.................................................................5.0mg
Lipoic acid .................................................................................5.0mg
Nicotinic acid.............................................................................5.0mg
Riboflavin ..................................................................................5.0mg
Thiamine·HCl ............................................................................5.0mg
Biotin.........................................................................................2.0mg
Folic acid ...................................................................................2.0mg
Preparation of Vitamin Solution:  Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Sparge
with 100% N 2 .
Preparation of Medium: Prepare and dispense medium under 80%
H 2 + 20% CO 2 . Add components to distilled/deionized water and bring
volume to 1.0L. Mix thoroughly. Adjust pH to 7.2. Sparge with 80%
H 2 + 20% CO 2 . Autoclave for 15 min at 15 psi pressure–121°C. Asep-
tically and anaerobically distribute into sterile tubes or bottles.
Use:  For the cultivation of Methanobacterium wolfei.