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Middlebrook 7H10 Agar with Middlebrook OADC Enrichment and Hemin (Hemin Medium for Mycobacterium)
[所属分类:培养基配方] [发布时间:2021-5-31] [发布人:网站管理员2] [阅读次数:] [返回]
Middlebrook 7H10 Agar
with Middlebrook OADC Enrichment and Hemin
(Hemin Medium for Mycobacterium)
Na 2 HPO 4 .......................................................................................1.5g
KH 2 PO 4 .........................................................................................1.5g
(NH 4 ) 2 SO 4 .....................................................................................0.5g
L- Glutamic acid.............................................................................0.5g
Sodium citrate...............................................................................0.4g
Ferric ammonium citrate.............................................................0.04g
MgSO 4 ·7H 2 O............................................................................0.025g
ZnSO 4 ·7H 2 O..............................................................................1.0mg
CuSO 4 ·5H 2 O..............................................................................1.0mg
Pyridoxine..................................................................................1.0mg
Biotin .........................................................................................0.5mg
CaCl 2 ·2H 2 O................................................................................0.5mg
Malachite Green.......................................................................0.25mg
Middlebrook OADC enrichment...........................................100.0mL
Glycerol .....................................................................................5.0mL
Hemin solution...........................................................................3.9mL
pH 6.6 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from BD Di-
agnostic Systems.
Middlebrook OADC Enrichment:
Composition per 100.0mL:
Bovine albumin fraction V ...........................................................5.0g
Glucose .........................................................................................2.0g
NaCl............................................................................................0.85g
Oleic acid....................................................................................0.05g
Catalase......................................................................................4.0mg
Preparation of Middlebrook OADC Enrichment: Add com-
ponents to distilled/deionized water and bring volume to 100.0mL.
Mix thoroughly. Filter sterilize.
Hemin Solution:
Composition per 100.0mL:
Hemin ...........................................................................................1.0g
NaOH (1N solution).................................................................20.0mL
Preparation of Hemin Solution: Add hemin to 20.0mL of 1N
NaOH solution. Mix thoroughly. Bring volume to 100.0mL with distilled/
deionized water.
Preparation of Medium: Add glycerol to 891.1mL of distilled/de-
ionized water and add remaining components, except Middlebrook
OADC enrichment. Mix thoroughly. Gently heat and bring to boiling.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C.
Aseptically add 100.0mL of sterile Middlebrook OADC enrichment.
Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile
tubes.
Use: For the isolation, cultivation, and maintenance of Mycobacte-
rium species, including Mycobacterium tuberculosis. For the cultiva-
tion and maintenance of Mycobacterium haemophilum. Also used for
determining the antimicrobial susceptibility of mycobacteria.
with Middlebrook OADC Enrichment and Hemin
(Hemin Medium for Mycobacterium)
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Agar ............................................................................................15.0gNa 2 HPO 4 .......................................................................................1.5g
KH 2 PO 4 .........................................................................................1.5g
(NH 4 ) 2 SO 4 .....................................................................................0.5g
L- Glutamic acid.............................................................................0.5g
Sodium citrate...............................................................................0.4g
Ferric ammonium citrate.............................................................0.04g
MgSO 4 ·7H 2 O............................................................................0.025g
ZnSO 4 ·7H 2 O..............................................................................1.0mg
CuSO 4 ·5H 2 O..............................................................................1.0mg
Pyridoxine..................................................................................1.0mg
Biotin .........................................................................................0.5mg
CaCl 2 ·2H 2 O................................................................................0.5mg
Malachite Green.......................................................................0.25mg
Middlebrook OADC enrichment...........................................100.0mL
Glycerol .....................................................................................5.0mL
Hemin solution...........................................................................3.9mL
pH 6.6 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from BD Di-
agnostic Systems.
Middlebrook OADC Enrichment:
Composition per 100.0mL:
Bovine albumin fraction V ...........................................................5.0g
Glucose .........................................................................................2.0g
NaCl............................................................................................0.85g
Oleic acid....................................................................................0.05g
Catalase......................................................................................4.0mg
Preparation of Middlebrook OADC Enrichment: Add com-
ponents to distilled/deionized water and bring volume to 100.0mL.
Mix thoroughly. Filter sterilize.
Hemin Solution:
Composition per 100.0mL:
Hemin ...........................................................................................1.0g
NaOH (1N solution).................................................................20.0mL
Preparation of Hemin Solution: Add hemin to 20.0mL of 1N
NaOH solution. Mix thoroughly. Bring volume to 100.0mL with distilled/
deionized water.
Preparation of Medium: Add glycerol to 891.1mL of distilled/de-
ionized water and add remaining components, except Middlebrook
OADC enrichment. Mix thoroughly. Gently heat and bring to boiling.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C.
Aseptically add 100.0mL of sterile Middlebrook OADC enrichment.
Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile
tubes.
Use: For the isolation, cultivation, and maintenance of Mycobacte-
rium species, including Mycobacterium tuberculosis. For the cultiva-
tion and maintenance of Mycobacterium haemophilum. Also used for
determining the antimicrobial susceptibility of mycobacteria.
