Middlebrook 7H12 Medium

[所属分类:培养基配方] [发布时间:2021-5-31] [发布人:网站管理员2] [阅读次数:] [返回]

Middlebrook 7H12 Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 102.5mL:

Bovine serum albumin..................................................................0.5g
Casein hydrolyslate.......................................................................0.1g
Catalase..................................................................................... 4800U
14 C-Palmitic acid .....................................................................100μCi
Middlebrook 7H9 broth.........................................................100.0mL
Antibiotic solution.....................................................................2.5mL
pH 6.8 ± 0.1 at 25°C
Middlebrook 7H9 Broth:
Composition per liter:
Na 2 HPO 4 .......................................................................................2.5g
KH 2 PO 4 .........................................................................................1.0g
Monosodium glutamate................................................................0.5g
(NH 4 ) 2 SO 4 ....................................................................................0.5g
Sodium citrate...............................................................................0.1g
MgSO 4 ·7H 2 O..............................................................................0.05g
Ferric ammonium citrate.............................................................0.04g
CuSO 4 ·5H 2 O..............................................................................1.0mg
Pyridoxine..................................................................................1.0mg
ZnSO 4 ·7H 2 O..............................................................................1.0mg
Biotin.........................................................................................0.5mg
CaCl 2 ·2H 2 O ...............................................................................0.5mg
Glycerol .....................................................................................2.0mL
Preparation of Middlebrook 7H9 Broth:  Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Antibiotic Solution:
Composition per 5.0mL:
Nalidixic acid................................................................................0.2g
Azlocillin......................................................................................0.1g
Amphotericin B ..........................................................................0.05g
Trimethoprim..............................................................................0.05g
Polymyxin B........................................................................500,000U
Preparation of Antibiotic Solution:  Add components to distilled/
deionized water and bring volume to 5.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium:  To 100.0mL of Middlebrook 7H9 broth,
add remaining components, except antibiotic solution. Mix thoroughly.
Filter sterilize. Aseptically distribute into bottles in 4.0mL volumes.
Prior to inoculation, aseptically add 0.1mL of antibiotic solution to
each bottle. Mix thoroughly.
Use: For the cultivation of Mycobacterium species from the blood of
patients suspected of having mycobacteremia.