MIL Medium (Motility Indole Lysine Medium)

[所属分类:培养基配方] [发布时间:2021-5-31] [发布人:网站管理员2] [阅读次数:] [返回]

MIL Medium
(Motility Indole Lysine Medium)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Peptone........................................................................................10.0g
Pancreatic digest of casein..........................................................10.0g
L- Lysine·HCl...............................................................................10.0g
Yeast extract..................................................................................3.0g
Agar ..............................................................................................2.0g
Dextrose........................................................................................1.0g
Ferric ammonium citrate...............................................................0.5g
Bromcresol Purple ......................................................................0.02g
pH 6.6 ± 0.2 at 25°C
Source: This medium is a vailable as a premixed powder and prepared
medium from BD Diagnostic Systems.
Preparation of Medium:  Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes in 5.0mL volumes. Autoclave for 15
min at 15 psi pressure–121°C.
Use: For the cultivation and differentiation of members of the Enter-
obacteriaceae on the basis of motility, lysine decarboxylase activity,
lysine deaminase activity, and indole production.