Milk Agar

[所属分类:培养基配方] [发布时间:2021-5-31] [发布人:网站管理员2] [阅读次数:] [返回]

Milk Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Mixture A...............................................................................500.0mL
Mixture B...............................................................................500.0mL
Mixture A:
Composition per 500.0mL:
Instant nonfat milk....................................................................100.0g
Preparation of Mixture A: Add instant nonfat milk to distilled/de-
ionized water and bring volume to 500.0mL. Mix thoroughly. Auto-
clave for 15 min at 15 psi pressure–121°C. Cool rapidly to 55°C.
Mixture B:
Composition per 500.0mL:
Agar............................................................................................15.0g
Nutrient broth..............................................................................12.5g
NaCl..............................................................................................2.5g
Preparation of Mixture B:  Add components to distilled/deionized
water and bring volume to 500.0mL. Mix thoroughly. Gently heat and
bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool
rapidly to 55°C.
Preparation of Medium: Aseptically combine cooled, sterile mix-
ture A with cooled, sterile mixture B. Mix thoroughly. Pour into sterile
Petri dishes in 20.0mL volumes.
Use:  For the cultivation and estimation of the numbers of Pseudomo-
nas aeruginosa in water by the membrane filter method.