Milk HiVeg Agar (Brown and Scott Modified)

[所属分类:培养基配方] [发布时间:2021-5-31] [发布人:网站管理员2] [阅读次数:] [返回]

Milk HiVeg Agar
(Brown and Scott Modified)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Instant nonfat milk....................................................................100.0g
Agar ............................................................................................15.0g
Plant peptone.................................................................................5.0g
NaCl..............................................................................................5.0g
Plant extract ..................................................................................1.5g
Yeast extract..................................................................................1.5g
pH 7.2 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Preparation of Medium: Add components, except milk, to dis-
tilled/deionized water and bring volume to 500.0mL. Mix thoroughly.
Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pres-
sure–121°C. Cool to 50°–55°C. Separately add nonfat milk to distilled/
deionized water and bring volume to 500.0mL. Mix thoroughly. Gently
heat and bring to boiling. Autoclave for 5 min at 15 psi pressure–
121°C. Cool to 50°–55°C. Aseptically combine the two sterile solu-
tions. Mix thoroughly. Pour into sterile Petri dishes or leave in tubes.
Use: For the confirmation of Pseudomonas aeruginosa in swimming
pool waters.