Mineral Medium with 2,4-Dichlorophenoxyacetic Acid

[所属分类:培养基配方] [发布时间:2021-6-1] [发布人:网站管理员2] [阅读次数:] [返回]

Mineral Medium with 2,4-Dichlorophenoxyacetic
Acid

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Na 2 HPO 4 ·12H 2 O...........................................................................9.0g
Ferric ammonium citrate...............................................................5.0g
MnSO 4 ·H 2 O..................................................................................3.0g
NH 4 Cl ...........................................................................................2.0g
KH 2 PO 4 .........................................................................................1.5g
MgSO 4 ·7H 2 O................................................................................0.2g
ZnSO 4 ·7H 2 O.................................................................................0.2g
Titriplex I.................................................................................10.0mg
CoSO 4 .......................................................................................10.0μg
2,4-Dichlorophenoxyacetic acid solution................................10.0mL
pH 7.0 ± 0.2 at 25°C
2,4-Dichlorophenoxyacetic Acid Solution:
Composition per 10.0mL:
2,4-Dichlorophenoxyacetic acid...................................................0.5g
Preparation of 2,4-Dichlorophenoxyacetic Acid Solution:
Add 2,4-dichlorophenoxyacetic acid to distilled/deionized water and
bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except 2,4-dichloro-
phenoxyacetic acid solution, to distilled/deionized water and bring vol-
ume to 990.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi
pressure–121°C. Aseptically add 10.0mL of sterile 2,4-dichlorophe-
noxyacetic acid solution. Mix thoroughly. Aseptically distribute into
sterile tubes or flasks.
Use: For the cultivation of bacteria that can utilize 2,4-dichlorophe-
noxyacetic acid as sole carbon source.