Mineral Medium with o-Nitrophenol (DSMZ Medium 461c)

[所属分类:培养基配方] [发布时间:2021-6-1] [发布人:网站管理员2] [阅读次数:] [返回]

Mineral Medium with o-Nitrophenol
(DSMZ Medium 461c)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

MgSO 4 ·7H 2 O................................................................................0.5g
NH 4 Cl ...........................................................................................0.3g
NaCl............................................................................................0.05g
CaCl 2 ..........................................................................................0.01g
MnSO 4 ........................................................................................0.01g
o-Nitrophenol solution...........................................................200.0mL
Phosphate solution.................................................................100.0mL
Trace elements solution SL-10..................................................1.0mL
pH 7.2 ± 0.2 at 25°C
Trace Elements Solution SL-10:
Composition per liter:
FeCl 2 ·4H 2 O...................................................................................1.5g
H 3 BO 3 ....................................................................................300.0mg
CoCl 2 ·6H 2 O...........................................................................190.0mg
MnCl 2 ·4H 2 O ..........................................................................100.0mg
ZnCl 2 ........................................................................................70.0mg
Na 2 MoO 4 ·2H 2 O .......................................................................36.0mg
NiCl 2 ·6H 2 O..............................................................................24.0mg
CuCl 2 ·2H 2 O ...............................................................................2.0mg
HCl (25% solution)....................................................................7.7mL
Preparation of Trace Elements Solution SL-10: Add
FeCl 2 ·4H 2 O to 10.0mL of HCl solution. Mix thoroughly. Add distilled/
deionized water and bring volume to 1.0L. Add remaining compo-
nents. Mix thoroughly. Sparge with 100% N 2 . Autoclave for 15 min at
15 psi pressure–121°C. Cool to room temperature.
Phosphate Solution:
Composition per 100.0mL:
Na 2 HPO 4 ·2H 2 O...........................................................................1.45g
KH 2 PO 4 .......................................................................................0.25g
Preparation of Phosphate Solution:  Add components to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temper-
ature.
o-Nitrophenol Solution:
Composition per liter:
o-Nitrophenol................................................................................0.5g
Preparation of o-Nitrophenol Solution:  Dissolve o-nitrophenol
in phosphate buffer (50 mM, pH 7.5) and bring volume to 1.0L. Steril-
ize by filtration.
Preparation of Medium:  Add components, except phosphate solu-
tion and o-nitrophenol solution, to distilled/deionized water and bring
volume to 700.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to room temperature. Aseptically add 100.0mL
phosphate solution and 200.0mL o-nitrophenol solution. Mix thor-
oughly. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation of o-nitrophenol-utilizing bacteria.