Mineral Medium with Phenol

[所属分类:培养基配方] [发布时间:2021-6-2] [发布人:网站管理员2] [阅读次数:] [返回]

Mineral Medium with Phenol

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Phenol...........................................................................................1.0g
K 2 HPO 4 .........................................................................................1.0g
NH 4 NO 3 ........................................................................................1.0g
(NH 4 ) 2 SO 4 ....................................................................................0.5g
MgSO 4 ..........................................................................................0.5g
KH 2 PO 4 .........................................................................................0.5g
NaCl..............................................................................................0.5g
CaCl 2 ...........................................................................................0.02g
FeSO 4 ..........................................................................................0.02g
Wolfe’s mineral solution..........................................................10.0mL
Wolfe’s Mineral Solution:
Composition per liter:
MgSO 4 ·7H 2 O................................................................................3.0g
Nitrilotriacetic acid.......................................................................1.5g
NaCl..............................................................................................1.0g
MnSO 4 ·H 2 O..................................................................................0.5g
FeSO 4 ·7H 2 O..................................................................................0.1g
CoCl 2 ·6H 2 O ..................................................................................0.1g
CaCl 2 .............................................................................................0.1g
ZnSO 4 ·7H 2 O.................................................................................0.1g
CuSO 4 ·5H 2 O...............................................................................0.01g
AlK(SO 4 ) 2 ·12H 2 O.......................................................................0.01g
H 3 BO 3 .........................................................................................0.01g
Na 2 MoO 4 ·2H 2 O ..........................................................................0.01g
Preparation of Wolfe’s Mineral Solution: Add nitrilotriacetic
acid to 500.0mL of distilled/deionized water. Dissolve by adjusting pH
to 6.5 with KOH. Add remaining components. Add distilled/deionized
water to 1.0L.
Preparation of Medium: Add components, except phenol, to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Au-
toclave for 15 min at 15 psi pressure–121°C. Aseptically add the
phenol. Mix thoroughly. Distribute into sterile tubes or flasks.
Use: For the cultivation and maintenance of Pseudomonas putida.