Mineral Medium with Phenylacetate

[所属分类:培养基配方] [发布时间:2021-6-2] [发布人:网站管理员2] [阅读次数:] [返回]

Mineral Medium with Phenylacetate

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Na 2 HPO 4 ·12H 2 O...........................................................................9.0g
Ferric ammonium citrate...............................................................5.0g
MnSO 4 ·H 2 O..................................................................................3.0g
NH 4 Cl ...........................................................................................2.0g
KH 2 PO 4 .........................................................................................1.5g
MgSO 4 ·7H 2 O................................................................................0.2g
ZnSO 4 ·7H 2 O.................................................................................0.2g
Titriplex I.................................................................................10.0mg
CoSO 4 .......................................................................................10.0μg
Phenylacetic acid solution .......................................................10.0mL
pH 7.0 ± 0.2 at 25°C
Phenylacetic Acid Solution:
Composition per 10.0mL:
Phenylacetic acid ..........................................................................0.5g
Preparation of Phenylacetic Acid Solution:  Add phenylacetic
acid to distilled/deionized water and bring volume to 10.0mL. Mix
thoroughly. Filter sterilize.
Preparation of Medium: Add components, except phenylacetic
acid solution, to distilled/deionized water and bring volume to
990.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–
121°C. Aseptically add 10.0mL of sterile phenylacetic acid solution.
Mix thoroughly. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation of bacteria that can utilize phenylacetic acid
as sole carbon source.