Mineral Medium with Sulfobenzoic Acid (DSMZ Medium 465e)

[所属分类:培养基配方] [发布时间:2021-6-2] [发布人:网站管理员2] [阅读次数:] [返回]

Mineral Medium with Sulfobenzoic Acid
(DSMZ Medium 465e)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Na 2 HPO 4 ·2H 2 O.............................................................................3.5g
KH 2 PO 4 .........................................................................................1.0g
(NH 4 ) 2 SO 4 .....................................................................................0.5g
MgCl 2 ·6H 2 O..................................................................................0.1g
Ca(NO 3 ) 2 ·4H 2 O...........................................................................0.05g
2-Sulfobenzoic acid .................................................................50.0mL
Vitamin solution.........................................................................2.5mL
Trace elements solution SL-4 ....................................................1.0mL
pH 7.25 ± 0.2 at 25°C
Trace Elements Solution SL-4:
Composition per liter:
EDTA............................................................................................0.5g
FeSO 4 ·7H 2 O..................................................................................0.2g
Trace elements solution SL-6 ................................................100.0mL
Trace Elements Solution SL-6:
Composition per liter:
H 3 BO 3 ...........................................................................................0.3g
CoCl 2 ·6H 2 O ..................................................................................0.2g
ZnSO 4 ·7H 2 O.................................................................................0.1g
MnCl 2 ·4H 2 O................................................................................0.03g
Na 2 MoO 4 ·H 2 O ............................................................................0.03g
NiCl 2 ·6H 2 O.................................................................................0.02g
CuCl 2· ·2H 2 O................................................................................0.01g
Preparation of Trace Elements Solution SL-6: Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Adjust pH to 3.4.
Preparation of Trace Elements Solution SL-4: Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Vitamin Solution:
Composition per 100.0mL:
Pyridoxamine.............................................................................5.0mg
Vitamin B 12 ................................................................................2.0mg
Nicotinic acid.............................................................................2.0mg
Thiamine-HCl·2H 2 O..................................................................1.0mg
p-Aminobenzoate.......................................................................1.0mg
Ca-pantothenate.........................................................................0.5mg
Biotin .........................................................................................0.2mg
Preparation of Vitamin Solution: Add components to distilled/
deionized water and bring volume to 100.0mL. Mix thoroughly. Filter
sterilize.
2-Sulfobenzoic Acid Solution:
Composition per 100.0mL:
2-Sulfobenzoic acid ......................................................................2.0g
Preparation of 2-Sulfobenzoic Acid Solution: Add 2-sulfoben-
zoic acid to 100.0mL distilled/deionized water. Mix thoroughly. Filter
sterilize.
Preparation of Medium:  Add components, except vitamin solution
and 2-sulfobenzoic acid solution, to 947.5mL distilled/deionized water.
Adjust pH to 7.25. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to room temperature. Aseptically add 2-sulfobenzoic acid solution and
vitamin solution. Mix thoroughly. Aseptically distribute to sterile tubes
or flasks.
Use: For the cultivation of sulfobenzoic acid-utilizing bacteria.