Mineral Medium with Vitamins

[所属分类:培养基配方] [发布时间:2021-6-2] [发布人:网站管理员2] [阅读次数:] [返回]

Mineral Medium with Vitamins

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1002.5mL:

Mineral medium...................................................................1000.0mL
Schlegel’s vitamin solution........................................................2.5mL
Mineral Medium:
Composition per 1010.0mL:
Na 2 HPO 4 .....................................................................................2.44g
KH 2 PO 4 .......................................................................................1.52g
(NH 4 ) 2 SO 4 ....................................................................................0.5g
MgSO 4 ·7H 2 O................................................................................0.2g
CaCl 2 ·2H 2 O ................................................................................0.05g
Trace elements solution SL-4..................................................10.0mL
pH 6.9 ± 0.2 at 25°C
Trace Elements Solution SL-4:
Composition per liter:
EDTA............................................................................................0.5g
FeSO 4 ·7H 2 O................................................................................. 0.2g
Trace elements solution SL-6................................................100.0mL
Trace Elements Solution SL-6:
Composition per liter:
MnCl 2 ·4H 2 O .................................................................................0.5g
H 3 BO 3 ...........................................................................................0.3g
CoCl 2 ·6H 2 O..................................................................................0.2g
ZnSO 4 ·7H 2 O................................................................................ 0.1g
Na 2 MoO 4 ·2H 2 O......................................................................... 0.03g
NiCl 2 ·6H 2 O.................................................................................0.02g
CuCl 2 ·2H 2 O................................................................................0.01g
Preparation of Trace Elements Solution SL-6: Add components

to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Trace Elements Solution SL-4:  Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Schlegel’s Vitamin Solution:
Composition per 100.0mL:
Nicotinic acid................................................................................2.0g
Pyridoxamine.............................................................................5.0mg
Cyanocobalamin ........................................................................2.0mg
p-Aminobenzoate.......................................................................1.0mg
Thiamine....................................................................................1.0mg
Calcium  DL -pantothenate...........................................................0.5mg
Biotin .........................................................................................0.2mg
Preparation of Schlegel’s Vitamin Solution : Add components
to distilled/deionized water and bring volume to 100.0mL. Filter ster-
ilize.
Preparation of Medium: Add components, except Schlegel’s vita-
min solution, to distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Asep-
tically add 2.5mL of sterile Schlegel’s vitamin solution. Mix thorough-
ly. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation of Pseudomonas chlororaphis and Rhodococ-
cus species.