Mitis Salivarius Agar

[所属分类:培养基配方] [发布时间:2021-6-3] [发布人:网站管理员2] [阅读次数:] [返回]

Mitis Salivarius Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Sucrose........................................................................................50.0g
Agar ............................................................................................15.0g
Enzymatic digest of protein........................................................10.0g
Proteose peptone.........................................................................10.0g
K 2 HPO 4 .........................................................................................4.0g
Dextrose........................................................................................1.0g
Trypan Blue ................................................................................0.08g
Crystal Violet.............................................................................0.8mg
Na 2 TeO 3 solution.......................................................................1.0mL
pH 7.0 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Na 2 TeO 3 Solution:
Composition per 10.0mL:
Na 2 TeO 3 ........................................................................................0.1g
Preparation of Na 2 TeO 3 Solution: Add Na 2 TeO 3 to 10.0mL of
distilled/deionized water. Mix thoroughly. Filter sterilize.
Caution: Potassium tellurite is toxic.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 999.0mL. Mix thoroughly. Gently heat and
bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool
medium to 50°–55°C. Aseptically add 1.0mL of the sterile Na 2 TeO 3
solution to the cooled basal medium. Mix thoroughly. Pour into sterile
Petri dishes or distribute into sterile tubes.
Use: For the selective isolation of Streptococcus mitis, Streptococcus
salivarius, and other viridans streptococci and enterococci.