MJANHOX-NO 3 Medium with Supplement (DSMZ Medium 1000)

[所属分类:培养基配方] [发布时间:2021-6-3] [发布人:网站管理员2] [阅读次数:] [返回]

MJANHOX-NO 3 Medium with Supplement
(DSMZ Medium 1000)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl .............................................................................................3.0g
MgCl 2 ·6H 2 O .............................................................................0.418g
Na 2 SiO 3 .........................................................................................0.5g
NH 4 Cl ...........................................................................................0.4g
MgSO 4 ·7H 2 O..............................................................................0.34g
KH 2 PO 4 .......................................................................................0.14g
KCl..............................................................................................0.033
CaCl 2 ·2H 2 O ..............................................................................0.014g
Fe 2 (SO 4 ) 3 ·7H 2 O........................................................................0.005g
NiCl 2 ·6H 2 O............................................................................0.005mg
Na 2 SeO 3 ·5H 2 O.......................................................................0.005mg
Bicarbonate solution................................................................10.0mL
Nitrate solution........................................................................10.0mL
Thiosulfate solution.................................................................10.0mL
Trace elements solution.............................................................1.0mL
pH 7.7 ± 0.2 at 25°C
Thiosulfate Solution:
Composition per 10.0mL:
Na 2 S 2 O 3 ·5H 2 O..............................................................................2.4g
Preparation of Thiosulfate Solution:  Add Na 2 S 2 O 3 ·5H 2 O to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Sparge with 100% N 2 . Filter sterilize.
Bicarbonate Solution :
Composition per 10.0mL:
NaHCO 3 ........................................................................................1.5g
Preparation of Bicarbonate Solution:  Add NaHCO 3 to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Nitrate Solution :
Composition per 10.0mL:
NaNO 3 ..........................................................................................2.0g
Preparation of Nitrate Solution:  Add NaNO 3 to distilled/deion-
ized water and bring volume to 10.0mL. Mix thoroughly. Filter steril-
ize.
Trace Elements Solution:
Composition per liter:
Nitrilotriacetic acid.......................................................................1.5g
MnSO 4 ·2H 2 O................................................................................0.5g
CoSO 4 ·7H 2 O.................................................................................0.5g
ZnSO 4 ·7H 2 O...............................................................................0.18g
CuSO 4 ·5H 2 O...............................................................................0.01g
KAl(SO 4 ) 2 ·12H 2 O.......................................................................0.02g
H 3 BO 3 .........................................................................................0.01g
Na 2 MoO 4 ·4H 2 O..........................................................................0.01g
Preparation of Trace Elements Solution:  Add nitrilotriacetic
acid to 500.0mL of distilled/deionized water. Dissolve by adjusting pH
to 6.5 with KOH. Add remaining components. Add distilled/deionized
water to 1.0L. Mix thoroughly.
Preparation of Medium:  Add components, except thiosulfate so-
lution, bicarbonate solution, nitrate solution, and vitamin solution, to
distilled/deionized water and bring volume to 970.0mL. Mix thorough-
ly. Adjust the pH to 7.7. Autoclave for 15 min at 15 psi pressure–121°C.
Cool to room temperature. Aseptically add thiosulfate solution, bicar-
bonate solution, nitrate solution, and vitamin solution. Sparge with a
gas mixture of 80% H 2 + 20% CO 2 for 5 min. Compress gas mixture into
gas phase (> 80% volume of the tube or bottle) at 3 atm.
Use:  For the cultivation of Sulfurihydrogenibium subterraneum.