Modified Biebl and Pfennig's Medium (DSMZ Medium 1069)

[所属分类:培养基配方] [发布时间:2021-6-3] [发布人:网站管理员2] [阅读次数:] [返回]

Modified Biebl and Pfennig's Medium
(DSMZ Medium 1069)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl............................................................................................20.0g
Malate/pyruvate ...........................................................................3.0g
MgSO 4 ·7H 2 O................................................................................2.0g
KH 2 PO 4 .........................................................................................0.5g
Yeast extract .................................................................................0.4g
NH 4 Cl ........................................................................................0.34g
CaCl 2 ·2H 2 O.................................................................................0.15g
Ferric citrate solution.................................................................5.0mL
Trace elements solution SL-7 ....................................................1.0mL
Vitamin solution.........................................................................1.0mL
pH 6.8 ± 0.2 at 25°C
Vitamin Solution:
Composition per 10.0ml:
Vitamin B 12 ................................................................................0.1mg
Preparation of Vitamin Solution:  Add vitamin B 12  to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Ferric Citrate Solution :
Composition per 10.0mL:
Ferric citrate................................................................................0.01g
Preparation of Ferric Citrate Solution:  Add ferric citrate to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Trace Elements Solution SL-7:
Composition per 1001.0mL:
CoCl 2 ·6H 2 O...........................................................................200.0mg
MnCl 2 ·4H 2 O ..........................................................................100.0mg
ZnCl 2 ........................................................................................70.0mg
H 3 BO 3 ......................................................................................60.0mg
Na 2 MoO 4 ·2H 2 O.......................................................................40.0mg
CuCl 2 ·2H 2 O.............................................................................20.0mg
NiCl 2 ·6H 2 O..............................................................................20.0mg
HCl (25%)..................................................................................1.0mL
Preparation of Trace Elements Solution SL-7:  Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium:  Add components, except vitamin solu-
tion, to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Adjust the pH to 6.8. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to room temperature. Aseptically add the vitamin solution.
Mix thoroughly. Aseptically dispense into culture vessels.
Use:  For the cultivation of Rhodovulum imhoffii.