Modified Bile Esculin Azide Agar

[所属分类:培养基配方] [发布时间:2021-6-3] [发布人:网站管理员2] [阅读次数:] [返回]

Modified Bile Esculin Azide Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Casein enzymic hydrolysate.......................................................17.0g
Agar............................................................................................13.5g
Oxgall .........................................................................................10.0g
Yeast extract..................................................................................5.0g
NaCl..............................................................................................5.0g
Peptic digest of animal tissue .......................................................3.0g
Sodium citrate...............................................................................1.0g
Esculin..........................................................................................1.0g
Ferric ammonium citrate...............................................................0.5g
NaN 3 ...........................................................................................0.25g
pH 7.1 ± 0.2 at 25°C
Source: This medium is available from HiMedia.
Caution:  Sodium azide has a tendency to form explosive metal azides
with plumbing materials. It is advisable to use enough water to flush
off the disposables.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the selective isolation and enumeration of group D strepto-
cocci.