Moeller Decarboxylase Broth

[所属分类:培养基配方] [发布时间:2021-6-7] [发布人:网站管理员2] [阅读次数:] [返回]

Moeller Decarboxylase Broth

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Amino acid..................................................................................10.0g
Peptic digest of animal tissue........................................................5.0g
Beef extract...................................................................................5.0g
Glucose .........................................................................................0.5g
Bromcresol Purple ......................................................................0.01g
Cresol Red..................................................................................5.0mg
Pyridoxal....................................................................................5.0mg
pH 6.0 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Use  L- lysine,  L- arginine, or  L- orni-
thine. Mix thoroughly. Gently heat until dissolved. Distribute into
screw-capped tubes in 5.0mL volumes. Autoclave for 15 min at 15 psi
pressure–121°C. A slight precipitate may form in the ornithine broth.
Use: For the differentiation of Gram-negative enteric bacteria based on
the production of arginine dihydrolase, lysine decarboxylase, or ornithine
decarboxylase.