MPY Agar (Maltose Peptone Yeast Extract Medium) (ATCC Medium 518)

[所属分类:培养基配方] [发布时间:2021-6-8] [发布人:网站管理员2] [阅读次数:] [返回]

MPY Agar
(Maltose Peptone Yeast Extract Medium)
(ATCC Medium 518)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................10.0g
Maltose .........................................................................................2.0g
Peptone .........................................................................................2.0g
Yeast extract..................................................................................1.0g
Potassium phosphate buffer (1M, pH 7.5)...............................10.0mL
pH 7.5 ± 0.2 at 25°C
Preparation of Medium: Add components, except potassium
phosphate buffer, to distilled/deionized water and bring volume to
990.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Filter sterilize
potassium phosphate buffer. Aseptically add sterile potassium phos-
phate buffer to sterile, cooled basal medium. Distribute into sterile
tubes or flasks.
Use:  For the cultivation and maintenance of Spirochaeta aurantia.