MRS Agar (DeMan, Rogosa, Sharpe Agar)

[所属分类:培养基配方] [发布时间:2021-6-8] [发布人:网站管理员2] [阅读次数:] [返回]

MRS Agar
(DeMan, Rogosa, Sharpe Agar)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Glucose .......................................................................................20.0g
Peptone........................................................................................10.0g
Agar ............................................................................................10.0g
Beef extract...................................................................................8.0g
Sodium acetate·3H 2 O....................................................................5.0g
Yeast extract..................................................................................4.0g
K 2 HPO 4 .........................................................................................2.0g
Triammonium citrate ....................................................................2.0g
MgSO 4 ·7H 2 O................................................................................0.2g
MnSO 4 ·4H 2 O..............................................................................0.05g
Sorbitan monooleate..................................................................1.0mL
pH 6.2 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from Oxoid
Unipath.
Preparation of Medium:  Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation of lactic acid bacteria.