MRS with Fermented Wort

[所属分类:培养基配方] [发布时间:2021-6-8] [发布人:网站管理员2] [阅读次数:] [返回]

MRS with Fermented Wort

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Solution A..............................................................................800.0mL
Solution B..............................................................................200.0mL
pH 6.2 ± 0.2 at 25°C
Solution A:
Composition per liter:
Glucose .......................................................................................20.0g
Peptone........................................................................................10.0g
Agar ............................................................................................10.0g
Beef extract...................................................................................8.0g
Sodium acetate·3H 2 O....................................................................5.0g
Yeast extract..................................................................................4.0g
K 2 HPO 4 .........................................................................................2.0g
Triammonium citrate.....................................................................2.0g
MgSO 4 ·7H 2 O................................................................................0.2g
MnSO 4 ·4H 2 O..............................................................................0.05g
Sorbitan monooleate..................................................................1.0mL
Preparation of Solution A: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
50°–55°C.
Solution B:
Composition per liter:
Malt extract.................................................................................15.0g
Maltose......................................................................................12.75g
Dextrin........................................................................................2.75g
Glycerol ......................................................................................2.35g
K 2 HPO 4 .........................................................................................1.0g
NH 4 Cl ...........................................................................................1.0g
Pancreatic digest of gelatin.........................................................0.78g
Preparation of Solution B: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
room temperature. Inoculate with Saccharomyces cerevisiae to allow
wort to ferment. Incubate for 24–48 hr. Allow to cool to room temper-
ature. Filter through Whatman filter paper to remove solids. Filter ster-

ilize.

Preparation of Medium: Aseptically combine 800.0mL of sterile
solution A and 200.0mL of sterile solution B. Mix thoroughly. Asepti-
cally distribute into sterile tubes or flasks.
Use: For the cultivation of yeasts and fungi.