MS 4 Agar

[所属分类:培养基配方] [发布时间:2021-6-9] [发布人:网站管理员2] [阅读次数:] [返回]

MS 4 Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................15.0g
Glucose .........................................................................................2.0g
(NH 4 ) 2 SO 4 .....................................................................................1.0g
Seawater........................................................................................1.0L
Preparation of Medium:  Add agar to 500.0mL of natural seawater.
Mix thoroughly. Gently heat and bring to boiling. Add (NH 4 ) 2 SO 4 to
250.0mL of natural seawater. Mix thoroughly. Add glucose to
250.0mL of natural seawater. Mix thoroughly. Autoclave the three so-
lutions separately for 15 min at 15 psi pressure–121°C. Aseptically
combine the three sterile solutions. Pour into sterile Petri dishes or dis-
tribute into sterile tubes.
Use: For the isolation and cultivation of Cytophaga species, Herpeto-
siphon species, Saprospira species, and Flexithrix species.