MSL86 Medium (DSMZ Medium 1068)

[所属分类:培养基配方] [发布时间:2021-6-9] [发布人:网站管理员2] [阅读次数:] [返回]

MSL86 Medium
(DSMZ Medium 1068)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl............................................................................................10.0g
MgSO 4 ·7H 2 O................................................................................2.0g
NH 4 Cl ..........................................................................................1.0g
Na 2 SO 4 .........................................................................................1.0g
Yeast extract .................................................................................0.5g
CaCl 2 ·2H 2 O ..................................................................................0.1g
Resazurin .................................................................................0.5mg
Vitamin solution.......................................................................10.0mL
Sulfide solution........................................................................10.0mL
Cysteine solution .....................................................................10.0mL
Lactate solution........................................................................10.0mL
Trace element solution SL-10....................................................1.0mL
pH 7.5 ± 0.2 at 25°C
Sulfide Solution :
Composition per 10.0mL:
Na 2 S·9H 2 O..................................................................................0.25g
Preparation of Sulfide Solution: Add Na 2 S·9H 2 O to distilled/de-
ionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave
under 100% N 2 for 15 min at 15 psi pressure–121°C. Cool to room
temperature.
Cysteine Solution:
Composition per 10.0mL:
L- Cysteine-HCl·2H 2 O .................................................................0.25g
Preparation of Cysteine Solution: Add  L- cysteine-HCl·2H 2 O to
to distilled/deionized water and bring volume to 10.0mL. Mix thor-
oughly. Sparge with 100% N 2 . Filter sterilize.
Lactate Solution :
Composition per 10.0mL:
Sodium lactate...............................................................................2.5g
Preparation of Lactate Solution:  Add sodium lactate to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Trace Elements Solution SL-10:
Composition per liter:
MgSO 4 ·7H 2 O................................................................................3.0g
Nitrilotriacetic acid.......................................................................1.5g
NaCl..............................................................................................1.0g
MnSO 4 ·2H 2 O................................................................................0.5g
CoSO 4 ·7H 2 O...............................................................................0.18g
ZnSO 4 ·7H 2 O...............................................................................0.18g
CaCl 2 ·2H 2 O...................................................................................0.1g
FeSO 4 ·7H 2 O..................................................................................0.1g
NiCl 2 ·6H 2 O...............................................................................0.025g
KAl(SO 4 ) 2 ·12H 2 O.......................................................................0.02g
H 3 BO 3 .........................................................................................0.01g
Na 2 MoO 4 ·4H 2 O ..........................................................................0.01g
CuSO 4 ·5H 2 O...............................................................................0.01g
Na 2 SeO 3 ·5H 2 O...........................................................................0.3mg
Preparation of Trace Elements Solution SL-10:  Add nitrilotri-
acetic acid to 500.0mL of distilled/deionized water. Dissolve by adjust-
ing pH to 6.5 with KOH. Add remaining components. Add distilled/
deionized water to 1.0L. Mix thoroughly. Adjust pH to 7.0.
Vitamin Solution:
Composition per liter:
Pyridoxine-HCl........................................................................10.0mg
Thiamine-HCl·2H 2 O..................................................................5.0mg
Riboflavin ..................................................................................5.0mg
Nicotinic acid.............................................................................5.0mg
D -Ca-pantothenate......................................................................5.0mg
p-Aminobenzoic acid.................................................................5.0mg
Lipoic acid .................................................................................5.0mg
Biotin .........................................................................................2.0mg
Folic acid....................................................................................2.0mg
Vitamin B 12 ................................................................................0.1mg
Preparation of Vitamin Solution:  Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Sparge
with 80% H 2 + 20% CO 2 . Filter sterilize.
Preparation of Medium:  Add components, except lactate,
cysteine, sulfide, and vitamin solutions, to distilled/deionized water
and bring volume to 960.0mL. Mix thoroughly. Sparge wtih 100% N 2
gas for at least 45 min. Dispense the medium under same gas atmo-
sphere in culture vessels. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to room temperature. Aseptically add the lactate, cysteine,
sulfide, and vitamin solutions. Adjust pH of the completed medium to
7.5.
Use: For the cultivation of Desulfopila aestuarii.