MSV I Agar

[所属分类:培养基配方] [发布时间:2021-6-10] [发布人:网站管理员2] [阅读次数:] [返回]

MSV I Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Glucose.......................................................................................0.15g
MSV agar......................................................................................1.0L
pH 7.2–7.5 at 25°C
MSV Agar:
Composition per liter:
Agar............................................................................................12.0g
(NH 4 ) 2 SO 4 ....................................................................................0.5g
K 2 HPO 4 ....................................................................................... 0.11g
KH 2 PO 4 .....................................................................................0.085g
MgSO 4 ·7H 2 O..............................................................................0.05g
CaCl 2 ·2H 2 0 .................................................................................0.05g
EDTA.........................................................................................3.0mg
FeCl 3 ·H 2 O..................................................................................2.0mg
Vitamin mix...............................................................................1.0mL
Preparation of MSV Agar: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly.
Vitamin Mix:
Composition per 100.0mL:
Calcium pantothenate .................................................................0.01g
Niacin..........................................................................................0.01g
Pyridoxine...................................................................................0.01g
p-Aminobenzoic acid..................................................................0.01g
Cocarboxylase.............................................................................0.01g
Inositol........................................................................................0.01g
Thiamine.....................................................................................0.01g
Riboflavin ...................................................................................0.01g
Biotin .........................................................................................0.5mg
Cyanocobalamin ........................................................................0.5mg
Folic acid....................................................................................0.5mg
Preparation of Vitamin Mix: Add components to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly.
Preparation of Medium: To 1.0L of MSV agar, add glucose. Ad-
just pH to 7.2–7.5. Gently heat to boiling. Distribute into tubes or
flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile
Petri dishes or leave in tubes.
Use: For the isolation, cultivation, and enrichment of heterotrophic
strains of Thiothrix species from water and environmental sources.