MUD SF Broth Base

[所属分类:培养基配方] [发布时间:2021-6-10] [发布人:网站管理员2] [阅读次数:] [返回]

MUD SF Broth Base

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Tryptose ......................................................................................40.0g
KH 2 PO 4 .......................................................................................10.0g
D -Galactose...................................................................................2.0g
Tween 80 (polysorbate 80) ...........................................................1.5g
4-Methylumbelliferyl-β- D -glucoside (MUD).............................0.15g
Selective supplement solution A .............................................10.0mL
Selective supplement solution B................................................1.0mL
pH 7.5 ± 0.2 at 25°C
Source: This medium is available from HiMedia.
Selective Supplement Solution A:
Composition per 10.0mL:
Thallium acetate............................................................................2.0g
Nalidixic acid..............................................................................0.25g
Preparation of Selective Supplement Solution A:  Add compo-
nents to distilled/deionized water and bring volume to 10.0mL. Mix
thoroughly. Filter sterilize.
Selective Supplement Solution B:
Composition per 20.0mL:
2,3,5,Triphenyltetrazolium chloride .............................................0.2g
Preparation of Selective Supplement Solution B:  Add 0.2g of
2,3,5,triphenyltetrazolium chloride to distilled/deionized water and
bring volume to 20.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except selective sup-
plement solutions A and B, to distilled/deionized water and bring vol-
ume to 989.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 50°C. Aseptically add selective supplement
solutions A and B. Mix thoroughly. Aseptically distribute into sterile
tubes or flasks.
Use: For the detection and enumeration of intestinal enterococci in
surface and wastewater in accordance with ISO committee under ISO
7899-1:1998.