Mueller-Kauffmann Tetrathionate Broth

[所属分类:培养基配方] [发布时间:2021-6-10] [发布人:网站管理员2] [阅读次数:] [返回]

Mueller-Kauffmann Tetrathionate Broth

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1028.0mL:

Na 2 S 2 O 3 ......................................................................................40.7g
CaCO 3 .........................................................................................25.0g
Pancreatic digest of casein............................................................7.0g
Ox bile ........................................................................................4.75g
Soya peptone.................................................................................2.3g
NaCl..............................................................................................2.3g
Iodine solution.........................................................................19.0mL
Brilliant Green solution .............................................................9.5mL
Iodine Solution:
Composition per 100.0mL:
Iodine..........................................................................................20.0g
KI................................................................................................25.0g
Preparation of Iodine Solution:  Add the KI to approximately
5.0mL of distilled/deionized water. Mix thoroughly. Add the iodine.
Gently heat to dissolve. Bring volume to 100.0mL with distilled/deion-
ized water. Filter sterilize.
Brilliant Green Solution:
Composition per 100.0mL:
Brilliant Green ..............................................................................0.1g
Preparation of Brilliant Green Solution:  Add the Brilliant
Green to distilled/deionized water and bring volume to 100.0mL.
Mix thoroughly. Gently heat while stirring and bring to boiling.
Continue boiling for 30 min while stirring until dye has dissolved.
Filter sterilize. Store protected from light.
Preparation of Medium: Add components, except iodine solution
and Brilliant Green solution, to distilled/deionized water and bring vol-
ume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Do not
autoclave. Cool to 45°C. Prior to use, add 19.0mL of iodine solution
and 9.5mL of Brilliant Green solution. Mix thoroughly. Aseptically
distribute into sterile tubes or flasks.
Use: For the isolation and cultivation of Salmonella species from spec-
imens with a mixed flora.