MUG Nutrient Agar

[所属分类:培养基配方] [发布时间:2021-6-11] [发布人:网站管理员2] [阅读次数:] [返回]

MUG Nutrient Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................15.0g
Peptic digest of animal tissue .......................................................5.0g
NaCl..............................................................................................5.0g
Beef extract...................................................................................1.5g
Yeast extract..................................................................................1.5g
4-Methylumbelliferyl-β- D -glucuronide (MUG)...........................0.1g
pH 7.4 ± 0.2 at 25°C
Source: This medium is available from HiMedia.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the detection of Escherichia coli in water and food samples
by a fluorogenic procedure.