MUG Sorbitol Agar

[所属分类:培养基配方] [发布时间:2021-6-11] [发布人:网站管理员2] [阅读次数:] [返回]

MUG Sorbitol Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Peptic digest of animal tissue .....................................................17.0g
Agar............................................................................................13.5g
D -Sorbitol....................................................................................10.0g
NaCl..............................................................................................5.0g
Proteose peptone...........................................................................3.0g
Bile salts mixture..........................................................................1.5g
4-Methylumbelliferyl-β- D -glucuronide (MUG)...........................0.1g
Neutral Red.................................................................................0.03g
Crystal Violet............................................................................0.001g
pH 7.1 ± 0.2 at 25°C
Source: This medium is available from HiMedia.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the isolation and identification of enteropathogenic Escheri-
chia coli associated with infant diarrhea by fluorogenic method.