Mycobacterium Medium

[所属分类:培养基配方] [发布时间:2021-6-11] [发布人:网站管理员2] [阅读次数:] [返回]

Mycobacterium Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Noble agar...................................................................................15.0g
(NH 4 ) 2 SO 4 ....................................................................................1.0g
Na 2 HPO 4 .......................................................................................0.5g
KH 2 PO 4 .........................................................................................0.5g
MgSO 4 ..........................................................................................0.2g
FeSO 4 ·7H 2 O...............................................................................5.0mg
MnSO 4 .......................................................................................2.0mg
Liquid paraffin...........................................................................5.0mL
Preparation of Medium:  Add components, except agar, to dis-
tilled/deionized water and bring volume to 1.0L. Homogenize in a
blender. Add agar. Gently heat and bring to boiling. Distribute into
tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour
into sterile Petri dishes or leave in tubes.
Use:  For the cultivation and maintenance of Mycobacterium paraffin-
icum.