Mycobactosel™ Agar

[所属分类:培养基配方] [发布时间:2021-6-15] [发布人:网站管理员2] [阅读次数:] [返回]

Mycobactosel™ Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................13.5g
Bovine albumin fraction V...........................................................5.0g
Glucose.........................................................................................2.0g
Na 2 HPO 4 .......................................................................................1.5g
KH 2 PO 4 .........................................................................................1.5g
Pancreatic digest of casein............................................................1.0g
NaCl............................................................................................0.85g
(NH 4 ) 2 SO 4 ....................................................................................0.5g
Monosodium glutamate................................................................0.5g
Sodium citrate...............................................................................0.4g
MgSO 4 ·7H 2 O..............................................................................0.05g
Ferric ammonium citrate.............................................................0.04g
Pyridoxine..................................................................................1.0mg
ZnSO 4 ·7H 2 O..............................................................................1.0mg
CuSO 4 ·5H 2 O..............................................................................1.0mg
Biotin.........................................................................................0.5mg
CaCl 2 ·H 2 O .................................................................................0.5mg
Malachite Green.......................................................................0.25mg
Antibiotic solution...................................................................10.0mL
Catalase solution......................................................................10.0mL
Glycerol .....................................................................................5.0mL
Oleic acid.................................................................................0.06mL
pH 6.6 ± 0.2 at 25°C
Source:  This medium is available as a prepared medium from BD Di-
agnostic Systems.
Antibiotic Solution:
Composition per 10.0mL:
Cycloheximide............................................................................0.36g
Nalidixic acid..............................................................................0.02g
Lincomycin................................................................................2.0mg
Preparation of Antibiotic Solution: Add components to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Catalase Solution:
Composition per 10.0mL:
Catalase......................................................................................3.0mg
Preparation of Catalase Solution: Add catalase to distilled/de-
ionized water and bring volume to 10.0mL. Mix thoroughly. Filter ster-
ilize.
Preparation of Medium: Add components, except antibiotic solu-
tion and catalase solution, to distilled/deionized water and bring vol-
ume to 980.0mL. Mix thoroughly. Gently heat and bring to boiling.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Aseptically add sterile antibiotic solution and sterile catalase solution.
Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile
tubes.
Use: For the selective isolation of mycobacteria from specimens con-
taining mixed flora.