Mycobiotic Agar (Cycloheximide Chloramphenicol Agar)

[所属分类:培养基配方] [发布时间:2021-6-15] [发布人:网站管理员2] [阅读次数:] [返回]

Mycobiotic Agar
(Cycloheximide Chloramphenicol Agar)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................15.0g
Enzymatic hydrolysate of soybean meal ....................................10.0g
Glucose.......................................................................................10.0g
Cycloheximide..............................................................................0.5g
Chloramphenicol.........................................................................0.05g
pH 6.5 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Cool tubes quickly in a slanted position.
Use: For the selective isolation and cultivation of pathogenic fungi.