- 定做培养基/定制培养基
- 颗粒培养基
- 标准菌株生化鉴定试剂盒
- 预灌装即用型成品培养基
- 2025年版中国药典
- 促销/特价商品
- 院感/疾控/体外诊断/采样管
- 样品采集与处理(均质)产品
- 按标准检索培养基
- 模拟灌装用培养基
- 干燥粉末培养基
- 培养基添加剂/补充剂
- 生化反应鉴定管
- 染色液等配套产品
- 对照培养基/标准品
- 实验耗材与器具
- 生化试剂/化学试剂
- 菌种鉴定服务
培养基配方
您现在的位置： 网站首页 >> 培养基配方
MYP Agar Base, HiVeg with Egg Yolk and Polymyxin B (Phenol Red Egg Yolk Polymyxin Agar Base, HiVeg)
[所属分类:培养基配方] [发布时间:2021-6-16] [发布人:网站管理员2] [阅读次数:] [返回]
MYP Agar Base, HiVeg
with Egg Yolk and Polymyxin B
(Phenol Red Egg Yolk Polymyxin Agar Base, HiVeg)
D -Mannitol..................................................................................10.0g
Plant peptone...............................................................................10.0g
NaCl............................................................................................10.0g
Plant extract No. 1........................................................................1.0g
Phenol Red................................................................................0.025g
Egg yolk emulsion, 20%..........................................................10.0mL
Polymyxin B solution................................................................1.0mL
pH 7.1 ± 0.2 at 25°C
Source: This medium, without egg yolk and polymyxin B, is avail-
able as a premixed powder from HiMedia.
Egg Yolk Emulsion, 20%:
Composition per 100.0mL:
Chicken egg yolks............................................................................ 11
Whole chicken egg ............................................................................1
NaCl (0.9% solution)...............................................................80.0mL
Preparation of Egg Yolk Emulsion, 20%: Soak eggs with 1:100
dilution of saturated mercuric chloride solution for 1 min. Crack eggs
and separate yolks from whites. Mix egg yolks with 1 chicken egg.
Measure 20.0mL of egg yolk emulsion and add to 80.0mL of 0.9%
NaCl solution. Mix thoroughly. Filter sterilize. Warm to 45°–50°C.
Polymyxin B Solution:
Composition per 1.0mL:
Polymyxin B..............................................................................1.0mg
Preparation of Polymyxin B Solution: Add polymyxin B to dis-
tilled/deionized water and bring volume to 1.0mL. Mix thoroughly. Fil-
ter sterilize.
Preparation of Medium: Add components—except egg yolk emul-
sion, 20%, and polymyxin B solution—to distilled/deionized water and
bring volume to 100.0mL. Mix thoroughly. Gently heat and bring to boil-
ing. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Aseptically add 10.0mL of sterile egg yolk emulsion, 20%, and 1.0mL
of sterile polymyxin B solution. Mix thoroughly. Pour into sterile Petri
dishes.
with Egg Yolk and Polymyxin B
(Phenol Red Egg Yolk Polymyxin Agar Base, HiVeg)
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Agar ............................................................................................15.0gD -Mannitol..................................................................................10.0g
Plant peptone...............................................................................10.0g
NaCl............................................................................................10.0g
Plant extract No. 1........................................................................1.0g
Phenol Red................................................................................0.025g
Egg yolk emulsion, 20%..........................................................10.0mL
Polymyxin B solution................................................................1.0mL
pH 7.1 ± 0.2 at 25°C
Source: This medium, without egg yolk and polymyxin B, is avail-
able as a premixed powder from HiMedia.
Egg Yolk Emulsion, 20%:
Composition per 100.0mL:
Chicken egg yolks............................................................................ 11
Whole chicken egg ............................................................................1
NaCl (0.9% solution)...............................................................80.0mL
Preparation of Egg Yolk Emulsion, 20%: Soak eggs with 1:100
dilution of saturated mercuric chloride solution for 1 min. Crack eggs
and separate yolks from whites. Mix egg yolks with 1 chicken egg.
Measure 20.0mL of egg yolk emulsion and add to 80.0mL of 0.9%
NaCl solution. Mix thoroughly. Filter sterilize. Warm to 45°–50°C.
Polymyxin B Solution:
Composition per 1.0mL:
Polymyxin B..............................................................................1.0mg
Preparation of Polymyxin B Solution: Add polymyxin B to dis-
tilled/deionized water and bring volume to 1.0mL. Mix thoroughly. Fil-
ter sterilize.
Preparation of Medium: Add components—except egg yolk emul-
sion, 20%, and polymyxin B solution—to distilled/deionized water and
bring volume to 100.0mL. Mix thoroughly. Gently heat and bring to boil-
ing. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Aseptically add 10.0mL of sterile egg yolk emulsion, 20%, and 1.0mL
of sterile polymyxin B solution. Mix thoroughly. Pour into sterile Petri
dishes.
