Myxococcus Medium

[所属分类:培养基配方] [发布时间:2021-6-16] [发布人:网站管理员2] [阅读次数:] [返回]

Myxococcus Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................12.0g
Pancreatic digest of casein............................................................1.0g
Meat extract ..................................................................................1.0g
Glucose solution ......................................................................50.0mL
pH 7.2 ± 0.2 at 25°C
Glucose Solution:
Composition per 50.0mL:
Glucose .........................................................................................1.0g
Preparation of Glucose Solution: Add glucose to distilled/deion-
ized water and bring volume to 50.0mL. Mix thoroughly. Autoclave for
15 min at 15 psi pressure–121°C. Cool to 25°C.
Preparation of Medium: Add components, except glucose solu-
tion, to distilled/deionized water and bring volume to 950.0mL. Mix
thoroughly. Adjust pH to 7.2. Gently heat and bring to boiling. Auto-
clave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Asepti-
cally add sterile glucose solution. Mix thoroughly. Pour into sterile
Petri dishes or distribute into sterile tubes or bottles. Allow tubes or
bottles to cool in a slanted position.
Use: For the cultivation of Myxococcus species.