Naphthalene Sulfonic Acid Medium

[所属分类:培养基配方] [发布时间:2021-6-16] [发布人:网站管理员2] [阅读次数:] [返回]

Naphthalene Sulfonic Acid Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1004.0mL:

Na 2 HPO 4 ·2H 2 O.............................................................................3.5g
KH 2 PO 4 .........................................................................................1.0g
NH 4 Cl .........................................................................................0.31g
MgCl 2 ·6H 2 O .................................................................................0.1g
Ca(NO 3 ) 2 ·4H 2 O ..........................................................................0.05g
Solution A..............................................................................100.0mL
Solution B..................................................................................3.0mL
Trace elements solution SL-4....................................................1.0mL
pH 7.0 ± 0.2 at 25°C
Solution A:
Composition per liter:
Glucose.........................................................................................3.0g
Glycerol ........................................................................................3.0g
Sodium succinate..........................................................................3.0g
Preparation of Solution A : Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Filter sterilize.
Solution B:
Composition per liter:
Naphthalene sulfonic acid.............................................................2.3g
Preparation of Solution B : Add naphthalene sulfonic acid to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter
sterilize.
Trace Elements Solution SL-4:
Composition per liter:
EDTA............................................................................................0.5g
FeSO 4 ·7H 2 O................................................................................. 0.2g
Trace elements solution SL-6................................................100.0mL
Preparation of Trace Elements Solution SL-4:  Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Filter sterilize.
Trace Elements Solution SL-6:
Composition per liter:
MnCl 2 ·4H 2 O..................................................................................0.5g
H 3 BO 3 ...........................................................................................0.3g
CoCl 2 ·6H 2 O ..................................................................................0.2g
ZnSO 4 ·7H 2 O................................................................................ 0.1g
Na 2 MoO 4 ·2H 2 O ......................................................................... 0.03g
NiCl 2 ·6H 2 O.................................................................................0.02g
CuCl 2 ·2H 2 O ................................................................................0.01g
Preparation of Trace Elements Solution SL-6: Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components, except solution A, so-
lution B, and trace elements solution SL-4, to distilled/deionized water
and bring volume to 900.0mL. Mix thoroughly. Autoclave for 15 min
at 15 psi pressure–121°C. Aseptically add 100.0mL of sterile solution
A, 3.0mL of sterile solution B, and 1.0mL of sterile trace elements so-
lution SL-4. Mix thoroughly. Aseptically distribute into sterile tubes or
flasks.
Use: For the cultivation of Pseudomonas putida.