Nelson Culture Medium for Naegleria

[所属分类:培养基配方] [发布时间:2021-6-17] [发布人:网站管理员2] [阅读次数:] [返回]

Nelson Culture Medium for Naegleria

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 100.0mL:

Glucose.......................................................................................0.17g
Panmede......................................................................................0.17g
Na 2 HPO 4 ..................................................................................14.2mg
KH 2 PO 4 ....................................................................................13.6mg
NaCl.........................................................................................12.0mg
CaCl 2 ·2H 2 O ...............................................................................0.4mg
MgSO 4 ·7H 2 O.............................................................................0.4mg
Bovine serum, heat-inactivated fetal .......................................10.0mL
Source: Panmede is available from Paines and Byrne Ltd., Greenford,
England, and Harrisons and Crosfield, Inc., Bronxville, NY.
Preparation of Medium: Add components, except bovine serum,
to distilled/deionized water and bring volume to 90.0mL. Mix thor-
oughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.
Aseptically add 10.0mL of sterile, heat-inactivated fetal bovine serum.
Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use
immediately.
Use: For the cultivation of Naegleria fowleri and Paratetramitus jugo-
sus.