Nelson Medium for Naegleria fowleri

[所属分类:培养基配方] [发布时间:2021-6-17] [发布人:网站管理员2] [阅读次数:] [返回]

Nelson Medium for Naegleria fowleri

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Glucose .........................................................................................1.0g
Ox liver digest...............................................................................1.0g
Page’s amoeba saline....................................................................1.0L
Fetal calf serum, inactivated....................................................20.0mL
Page’s Amoeba Saline:
Composition per liter:
Na 2 HPO 4 ...................................................................................0.142g
KH 2 PO 4 .....................................................................................0.136g
NaCl............................................................................................0.12g
MgSO 4 ·7H 2 O.............................................................................4.0mg
CaCl 2 ·2H 2 O................................................................................4.0mg
Preparation of Page’s Amoeba Saline: Add components to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Preparation of Medium: Add the glucose and ox liver digest to
1.0L of Page’s amoeba saline. Mix thoroughly. Distribute into screw-
capped tubes in 10.0mL volumes. Autoclave for 15 min at 15 psi pres-
sure–121°C. Cool to 25°C. Aseptically add 0.2mL of sterile fetal calf
serum to each tube. Mix thoroughly.
Use: For the cultivation of Naegleria fowleri.