Neomycin Blood Agar

[所属分类:培养基配方] [发布时间:2021-6-17] [发布人:网站管理员2] [阅读次数:] [返回]

Neomycin Blood Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein..........................................................14.5g
Agar ............................................................................................14.0g
Papaic digest of soybean meal......................................................5.0g
NaCl..............................................................................................5.0g
Growth factors ..............................................................................1.5g
Sheep blood, defibrinated........................................................50.0mL
Neomycin solution...................................................................10.0mL
pH 7.3 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Neomycin Solution:
Composition per 10.0mL:
Neomycin sulfate........................................................................0.03g
Preparation of Neomycin Solution: Add neomycin sulfate to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components, except sheep blood and
neomycin solution, to distilled/deionized water and bring volume to
940.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically
add sterile sheep blood and sterile neomycin solution. Mix thoroughly.
Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the isolation and cultivation of group A streptococci (Strep-
tococcus pyogenes) and group B streptococci (Streptococcus agalac-
tiae) from throat cultures and other clinical specimens.