Neomycin Medium No. 1

[所属分类:培养基配方] [发布时间:2021-6-17] [发布人:网站管理员2] [阅读次数:] [返回]

Neomycin Medium No. 1

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................15.0g
Peptone .........................................................................................5.0g
NaCl..............................................................................................5.0g
Yeast extract..................................................................................2.0g
Beef extract...................................................................................1.0g
Sucrose solution.......................................................................20.0mL
Neomycin solution...................................................................10.0mL
pH 7.0 ± 0.1 at 25°C
Sucrose Solution:
Composition per 100.0mL:
Sucrose..........................................................................................2.5g
Preparation of Sucrose Solution: Add sucrose to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster-
ilize.
Neomycin Solution:
Composition per 10.0mL:
Neomycin sulfate...................................................................500.0mg
Preparation of Neomycin Solution: Add neomycin sulfate to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components, except sucrose solution
and neomycin solution, to distilled/deionized water and bring volume to
970.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for
15 min at 15 psi pressure–121°C. Aseptically add 20.0mL of sterile su-
crose solution and 10.0mL of sterile neomycin solution. Mix thoroughly.
Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation of Micrococcus luteus and Pseudomonas aerug-
inosa.