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Nitrate Assimilation Medium, Auxanographic Method for Yeast Identification
[所属分类:培养基配方] [发布时间:2021-6-18] [发布人:网站管理员2] [阅读次数:] [返回]
Nitrate Assimilation Medium,
Auxanographic Method for Yeast Identification
Glucose.......................................................................................10.0g
KH 2 PO 4 .........................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.5g
NaCl..............................................................................................0.1g
CaCl 2 ·2H 2 O ..................................................................................0.1g
DL- Methionine.............................................................................0.02g
DL- Tryptophan.............................................................................0.02g
L- Histidine·HCl...........................................................................0.01g
Inositol.......................................................................................2.0mg
H 3 BO 3 ........................................................................................0.5mg
ZnSO 4 ·7H 2 O..............................................................................0.4mg
MnSO 4 ·4H 2 O.............................................................................0.4mg
Thiamine·HCl ............................................................................0.4mg
Pyridoxine..................................................................................0.4mg
Niacin.........................................................................................0.4mg
Calcium pantothenate ................................................................0.4mg
p-Aminobenzoic acid.................................................................0.2mg
Riboflavin ..................................................................................0.2mg
FeCl 3 ..........................................................................................0.2mg
Na 2 MoO 4 ·4H 2 O .........................................................................0.2mg
KI...............................................................................................0.1mg
CuSO 4 ·5H 2 O............................................................................0.04mg
Folic Acid ...................................................................................2.0μg
Biotin ..........................................................................................2.0μg
pH 4.5 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into screw-capped tubes in 20.0mL volumes. Au-
toclave for 15 min at 15 psi pressure–121°C.
Use: For nitrate assimilation tests by the auxanographic method.
Auxanographic Method for Yeast Identification
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Noble agar...................................................................................20.0gGlucose.......................................................................................10.0g
KH 2 PO 4 .........................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.5g
NaCl..............................................................................................0.1g
CaCl 2 ·2H 2 O ..................................................................................0.1g
DL- Methionine.............................................................................0.02g
DL- Tryptophan.............................................................................0.02g
L- Histidine·HCl...........................................................................0.01g
Inositol.......................................................................................2.0mg
H 3 BO 3 ........................................................................................0.5mg
ZnSO 4 ·7H 2 O..............................................................................0.4mg
MnSO 4 ·4H 2 O.............................................................................0.4mg
Thiamine·HCl ............................................................................0.4mg
Pyridoxine..................................................................................0.4mg
Niacin.........................................................................................0.4mg
Calcium pantothenate ................................................................0.4mg
p-Aminobenzoic acid.................................................................0.2mg
Riboflavin ..................................................................................0.2mg
FeCl 3 ..........................................................................................0.2mg
Na 2 MoO 4 ·4H 2 O .........................................................................0.2mg
KI...............................................................................................0.1mg
CuSO 4 ·5H 2 O............................................................................0.04mg
Folic Acid ...................................................................................2.0μg
Biotin ..........................................................................................2.0μg
pH 4.5 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into screw-capped tubes in 20.0mL volumes. Au-
toclave for 15 min at 15 psi pressure–121°C.
Use: For nitrate assimilation tests by the auxanographic method.
