Nutrient Agar pH 9.5

[所属分类:培养基配方] [发布时间:2021-6-22] [发布人:网站管理员2] [阅读次数:] [返回]

Nutrient Agar pH 9.5

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................15.0g
Pancreatic digest of gelatin...........................................................5.0g
Beef extract...................................................................................3.0g
Na 2 CO 3  solution..................................................................... variable
pH 9.5–10.0 at 25°C
Na 2 CO 3 Solution:
Composition per 100.0mL:
Na 2 CO 3  .........................................................................................5.0g
Preparation of Na 2 CO 3 Solution: Add Na 2 CO 3  to distilled/de-
ionized water and bring volume to 100.0mL. Mix thoroughly. Auto-
clave for 15 min at 15 psi pressure–121°C. Cool to 50°C.
Preparation of Medium: Add components, except Na 2 CO 3  solu-
tion, to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C. Adjust pH to 9.5 with sterile
Na 2 CO 3  solution. Mix thoroughly. Pour into sterile Petri dishes or dis-
tribute into sterile tubes. Allow tubes to cool in a slanted position.
Use: For the cultivation and maintenance of Bacillus alcalophilus.