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Nutrient Agar with Dihydrostreptomycin

[所属分类:培养基配方] [发布时间:2021-6-22] [发布人:网站管理员2] [阅读次数:] [返回]
Nutrient Agar with Dihydrostreptomycin

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................15.0g
Pancreatic digest of gelatin...........................................................5.0g
Beef extract...................................................................................3.0g
Dihydrostreptomycin solution .................................................10.0mL
pH 6.8 ± 0.2 at 25°C
Source:  Nutrient agar is available as a premixed powder from BD Di-
agnostic Systems.
Dihydrostreptomycin Solution:
Composition per 10.0mL:
Dihydrostreptomycin................................................................0.625g
Preparation of Dihydrostreptomycin Solution: Add dihy-
drostreptomycin to distilled/deionized water and bring volume to
10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except dihydrostrepto-
mycin solution, to distilled/deionized water and bring volume to
990.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically
add sterile dihydrostreptomycin solution. Mix thoroughly. Pour into
sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation and maintenance of Escherichia coli, Micro-
coccus luteus, Shigella species, and Vibrio cholerae.
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