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Nutrient Agar with Erythromycin

[所属分类:培养基配方] [发布时间:2021-6-23] [发布人:网站管理员2] [阅读次数:] [返回]
Nutrient Agar with Erythromycin

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................15.0g
Peptone .........................................................................................5.0g
Beef extract...................................................................................3.0g
Erythromycin solution.............................................................10.0mL
pH 6.8 ± 0.2 at 25°C.
Erythromycin Solution:
Composition per 10.0mL:
Erythromycin................................................................................0.6g
Preparation of Erythromycin Solution: Add erythromycin to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Filter sterilize.
Preparation of Medium: Add components, except erythromycin
solution, to distilled/deionized water and bring volume to 990.0mL.
Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min
at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile
erythromycin solution. Mix thoroughly. Pour into sterile Petri dishes or
distribute into sterile tubes.
Use:  For the cultivation and maintenance of Micrococcus luteus.
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