Nutrient Agar with 1% Methanol (ATCC Medium 620)

[所属分类:培养基配方] [发布时间:2021-6-23] [发布人:网站管理员2] [阅读次数:] [返回]

Nutrient Agar with 1% Methanol
(ATCC Medium 620)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................15.0g
Pancreatic digest of gelatin...........................................................5.0g
Beef extract...................................................................................3.0g
Methanol..................................................................................10.0mL
pH 6.8 ± 0.2 at 25°C
Source:  Nutrient agar is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Filter sterilize methanol. Add compo-
nents, except methanol, to distilled/deionized water and bring volume
to 990.0mL. Mix thoroughly. Gently heat and bring to boiling. Auto-
clave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Asepti-
cally add sterile methanol. Mix thoroughly. Pour into sterile Petri
dishes or distribute into sterile tubes.
Use: For the cultivation and maintenance of Bacillus species, Methyl-
omonas clara, and Pseudomonas methanolica.